the first report of stenotrophomonas sp. associated with canker and leaf spot disease of stone fruits in iran
نویسندگان
چکیده
department of plant protection, faculty of agriculture, vali-e-asr university of rafsanjan. *.corresponding author, [email protected] in september of 2012 some bacterial strains were isolated from peach (prunuspersica),apricot (prunusarmeniaca)and sour cheery (prunuscerasus)trees with leaf spot, canker and dieback symptoms on twig and stem in alborz province. the symptoms were similar to those caused by pseudomonas syringaepv. syringae (pss) and xanthomonasarboricolapv. pruniwhich have been previously reported in north of iran (aldaghi, et al., 2010.;mahmoudiet al., 2012). the smooth, pale yellow and round, mucoid colonies were recovered from symptomatic tissues on sucrose nutrient agar (nas). for pathogenicity tests, ten detached young leaves of peach,apricot and cherry (prunus sp.)were separately placed on 1% agar plate and sprayed with strains isolated from infected tissues in iran and three type strains of x. a. pv. pruni(icmp17186), pss(atcc19310) and erwiniaamylovora(icmp1540) at 1×108 cfu/ml. bacteria were identified on the basis of the biochemical and molecular characteristic (palleroni, & bradbury., 1993; schaadet al., 2001).ice nucleation activity measured by the droplet freezing method.based on biochemical tests, none of isolated bacteria were identified as pss or x. a.pv. pruni. all strains induced a hypersensitive response in tobacco. representative strains were gram-negative, oxidative negativeand also negative for formation of levan and casein, hydrolysis of starch and tyrosine, the reaction of isolates forindole production, reducing substance from sucrose, production of urease, oxidase, lecithinase, arginine dehydrolase, reduction of nitrate, growth on 5% on nacl and acid production from, maltose, lactose and trehalose were negative. the isolates were positive for, production of h2s from cysteine and peptone, protease, hydrolysis of gelatin and growth at 37°c. induction of fast hypersensitive reaction on tobacco and geranium may be considered as the potential of the isolates in causing disease in one or more plant species. the leaves inoculated with isolates developed leaf spots similar to those observed in the field. the same bacteria were reisolated from symptomatic leaves and identification was confirmed. the 16s rdnawas amplified by pcr using primer pairs 63f and 1384r (marchesiet al., 1998). pcr products were sequenced and sequences were aligned and compared with those deposited in genbank. 1100 nucleotides of sequences of the strain number 106 (accession no.kf752591) and 81 (accession no.kf7440537) showed 99% identity with those of stenotrophomonas isolates. all of isolates were positive for ice nucleation. mostof ice nucleation active bacteria are epiphytic and exist almostin all plants. the ability of bacteria to form ice nucleus is very important. ice nucleation active bacteria can catalyze ice formation at temperatures as high as -2°c and have significant role in freezing injuries ofcold sensitive plants. the association of two species of this genus, including s. maltipholia and s. rhizophila with citrus blast havepreviously been reported from northern iran, and their icenucleation activity of demonstrated (alimiet al., 2012).in that study the possibility of pathogenicity of collected strains has not been evaluated.this is the first report on, association of stenotrophomonas sp.with canker and leaf spot disease of stone fruits tress in alborz province of iran.
منابع مشابه
the survey of the virtual higher education in iran and the ways of its development and improvement
این پژوهش با هدف "بررسی وضعیت موجود آموزش عالی مجازی در ایران و راههای توسعه و ارتقای آن " و با روش توصیفی-تحلیلی و پیمایشی صورت پذیرفته است. بررسی اسنادو مدارک موجود در زمینه آموزش مجازی نشان داد تعداد دانشجویان و مقاطع تحصیلی و رشته محل های دوره های الکترونیکی چندان مطلوب نبوده و از نظر کیفی نیز وضعیت شاخص خدمات آموزشی اساتید و وضعیت شبکه اینترنت در محیط آموزش مجازی نامطلوب است.
azerbaijans political development after the collapse of soviet union and implication on relation with the islamic republic of iran
در فصل اول این پایاین نامه در خصوص تاریخ کشور اذربایجان قبل و بعد از جدایی این کشور از ایران مورد بررسی قرار گرفته است ودر فصل دوم تحولات سیاسی این کشور بعد از 1991 و در واقع بعد از فروپاشی شوروی و دولتهایی که روی کار امدند در از جمله دولت ابولفضل ایلچی بیگ،دولت حیدر علی اف وبعد از او پسرش الهام علی اف و نگرش هرکدام از این دولتها به سیاسیت خارجی اذربایجان مورد اشاره قرار گرفته است.در ودر فصل سو...
survey microbial decomposition leaf litter of salix sp. populus sp. robinia sp. in artificial stream
مطالعه اکولوژیک از سرچشمه رودخانه قره سو(سراب روانسر) در استان کرمانشاه واقع در غرب ایران در پائیز 1378 انجام شد، حداکثر دمای آب 20 درجه سانتی گراد بود، هنگام مطالعه دما در سرچشمه 18 سانتی گراد بود، مقدار عناصر شیمیایی آب اندازه گیری نشد. در این پژوهش لاشبرگ های درختی از بید، چنار، اقاقیا جمع آوری سپس توسط بخش هرباریوم دانشکده کشاورزی دانشگاه شناسایی شدند. وزن خاکستر خشک afdm در(نمونه برداری ...
15 صفحه اولthe impact of e-readiness on ec success in public sector in iran the impact of e-readiness on ec success in public sector in iran
acknowledge the importance of e-commerce to their countries and to survival of their businesses and in creating and encouraging an atmosphere for the wide adoption and success of e-commerce in the long term. the investment for implementing e-commerce in the public sector is one of the areas which is focused in government‘s action plan for cross-disciplinary it development and e-readiness in go...
phylogeography and genetic diversity of the lesser mouse- eared bat (myotis blythii) in iran
in current study, 63 samples of bat populations collected from differ regions were used for evaluating the geographic variations. twenty cranial and dental characters for traditional morphometric and landmarks method on the ventral, dorsal skull and mandible for geometry morphometric studies were used. statistical analyses of traditional morphometric and geometry morphometric data indicated low...
منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
بیماریهای گیاهیجلد ۵۱، شماره ۱، صفحات ۱۰۳-۱۰۶
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023